Journal: AMB Express

Article Title: Celastrol boosts fluconazole efficacy against vaginal candidiasis: in vitro and in vivo evidence

doi: 10.1186/s13568-025-01824-6

Figure Lengend Snippet: The interactions of celastrol with the crystal structure of; CDR1-AF-Q5ANA3-F1-model_v1 represented by 2D A and 3D structure B , CDR2-AF-P78595-F1-model_v4 represented by 2D ( C ) and 3D structure ( D ), MDR1-AF-Q5ABU7-F1-model_v1 represented by 2D ( E ) and 3D structure ( F )

Article Snippet: The 3D structure of, UniProtKB - Q5ANA3 (CDR1_CANAL) Pleiotropic ABC efflux transporter of multiple drugs CDR1 encoded by CDR1 gene (Muzzey et al. ; van het Hoog et al. ) UniProtKB -P78595 (CDR2_CANAL) Multidrug resistance protein CDR2 encoded by CDR2 gene and UniProtKB – Q5ABU7 (MDR1_CANAL) Multidrug resistance protein 1 of efflux pump encoded by MDR1 gene (Goldway et al. ; Jones et al. ), that are normally expressed in C. albicans (strain SC5314 / ATCC MYA-2876)(Muzzey et al. ; Sanglard et al. ) were drawn in PDB format as AlphaFold predicted structures ( https://alphafold.ebi.ac.uk/ ).

Techniques:

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: The AS0326 antibody blocks MFAP4s interaction with endothelial integrins (A) HPMECs were subjected to MFAP4-mediated adhesion, and inhibition of adhesion was tested with integrin α V β 3 - or integrin α V β 5 -blocking antibodies. (B) mAS0326 antibody (mAS) blocks HPMEC adhesion to MFAP4. MFI is of fluorescently labeled cells. IC, isotype control. Significance is calculated relative to the MFAP4-treated positive control in (A) and (B). Flow cytometry staining of (C) integrin α V β 3 and (D) integrin α V β 5 in HPMEC compared to IC. Representative histograms of n = 3 independent experiments are shown. Human primary retinal endothelial cells (RECs) were seeded on immobilized albumin or MFAP4. RECs were stimulated with VEGF and 24-h proliferation was assessed. (E) REC proliferation was co-treated with integrin α V β 3 - or α V β 5 -blocking antibodies or hAS0326 (hAS). REC migration for 3.5 h on MFAP4-coated surface was assessed by Transwell assay using VEGF as chemoattractant. (F) MFAP4-dependent migration was treated with integrin α V β 3 - or α V β 5 -blocking antibodies or hAS0326. Data are shown as individual datapoints with mean (SD) of n = 3 independent experiments. Data are normalized to albumin control. Significance is calculated relative to the hAS0326 treatment group for (E) and (F). Significance calculations are performed using one-way ANOVA followed by Dunnett’s multiple comparison test. Representative images of REC’s (purple) migrated through the pores of the Transwell assay inserts when migration on (G) albumin-coated insert or (H) MFAP4-coated insert. Bar, 100 μm. All antibodies were provided in 10 μg/mL doses for (A), (B), (E), and (F).

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Inhibition, Blocking Assay, Labeling, Control, Positive Control, Flow Cytometry, Staining, Migration, Transwell Assay, Comparison

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: Specificity of the AS0326 antibody (A) mAS0326 and (B) hAS0326 efficiently detect MFAP4 in WT ( Mfap4 +/+ ) mouse serum, but not in MFAP4-deficient ( Mfap4 −/− ) mouse serum. (C) Increasing concentrations of hAS0326-Fab or hAS0326Y94A L-CDR3 Fab variant were applied for inhibition of 1.5 nM MFAP4 binding to an excess of immobilized integrin α V β 3 . (D–F) Brown symbols, HG-HYB7-5; purple symbols, mAS0326; red symbols; hAS0326. (D) Both mAS0326 and hAS0326 efficiently detect immobilized recombinant human MFAP4 (rhMFAP4 coating) variant carrying RGD-AAA mutation (RGD-AAA), while HG-HYB7-5 antibody show RGD-dependent binding. Both mAS0326 and hAS0326 efficiently detect immobilized (E) recombinant mouse (rm) MFAP4, and (F) rhMFAP4, while HG-HYB7-5 is rhMFAP4 specific. Data are shown as means (SD) of n = 3 independent experiments.

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Variant Assay, Inhibition, Binding Assay, Recombinant, Mutagenesis

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: Structural basis for the hAS0326 Fab interaction with MFAP4 The hAS0326 Fab complex and the MFAP4 octamer. (A) Cartoon representation of the octameric MFAP4-Fab complex. (B) Each Fab contacts a single MFAP4 monomer at an epitope next to the MFAP4 N terminus but far from the binding site for the Ca 2+ ion (cyan sphere). (C) The MFAP4 octamer is built from two FIBCD1-like tetramers (colored orange and red) with eight Ca 2+ ions located at the tetramer-tetramer interface. (D) Inside view showing the concave face of the tetramer. (E) Top view of the convex face of the tetramer with the four bound Fab molecules. Orange spheres mark the first MFAP4 residue that can be located. Presumably, the disordered RGD integrin-binding motif at the N terminus of MFAP4 is trapped and inaccessible inside the funnel-shaped space formed by the Fab molecules. Nt, N terminus. (F) The epitope mapped on MFAP4. Residues primarily in contact with heavy-chain and light-chain CDRs are colored blue and green, respectively. (G) Details of the intermolecular interaction centered on MFAP4 residues 24–29. Putative polar interactions are indicated by dotted lines.

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Binding Assay, Residue

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: hAS0326 inhibits retinal vascular leakage in laser-induced mouse CNV and STZ-induced rat retinopathy (A) i.v.t. hAS0326 injection was performed on day 0 and 7 after laser burn in a 14-day mouse laser-induced CNV model. Representative (B) FFA and (C) IB4-stained images on laser-induced lesions from the different treatment groups. (D) Quantification of lesion size by FFA or (E) vascular volume (IB4-positive volume). Each data point represents a mean of 1–4 lesions per eye, n = 4–13 animals/group. (F) Type 1 diabetes was induced in Norway brown rats following a dose of STZ (50 mg/kg) and animals were maintained for 21 days. i.v.t. hAS0326 injection was performed on day 0 and 7. (G) FFA was used to calculate the vascular permeability coefficient in a 21-day time course after induction of diabetes. (H) The vascular permeability coefficient day 21. n = 7–8 animals/group (one eye per animal). Individual data points are shown with mean (SD) for all animal experiments. Significance is calculated relative to saline treatment. Significance calculations are performed using one-way ANOVA followed by Dunnett’s multiple comparison test.

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Injection, Staining, Permeability, Saline, Comparison

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: i.v.t. hAS0326 reduces DL-AAA-induced retinal vascular leakage area in non-human primates (A) i.v.t. DL-AAA was provided in week 0 in each eye of African green monkeys. Eyes were randomized into treatment groups and 50 μL of vehicle, ranibizumab (positive control, 0.5 mg), or hAS0326 (2 mg) was administered i.v.t. by week 10. FFA imaging was obtained at week 8 post DL-AAA administration (pre-dose) and eight times following i.v.t. treatments (weeks 10, 12, 13, 14, 16, 18, 20, and 22). Fluorescein leakage was assessed by computer-based analysis of 1-min FFA intensity. (B) Vitreous MFAP4 levels are shown as means (SD) from week 0–22. Significance is calculated for the difference in MFAP4 levels at individual time points relative to week 0 in the two treatment groups. Significance was calculated by mixed-effects analysis followed by Dunnett’s multiple comparison test. Nd, not detected. (C) Representative FFA images obtained in week 10 (pre-dose), week 12 (maximal positive control efficacy), and week 22 (end of study) are shown for each treatment group. (D–F) Relative FFA-defined leakage areas in percentage of pre-dose area are shown as means (SD) from week 10–22 for vehicle, ranibizumab, and hAS0326 treatment, respectively. Data are normalized to the pre-dose leakage area. Significance is calculated for the difference in leakage from individual time points relative to week 10 in the respective treatment groups. Significance is estimated by mixed-effects analysis followed by Dunnett’s multiple comparison test. Relative leakage areas in percentage of pre-dose area (G) week 12 or (H) week 22. Individual data points are shown with mean (SD). Significance calculations are performed using one-way ANOVA followed by Tukey’s multiple comparison test. n = 6–8 eyes/group.

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Positive Control, Imaging, Comparison

Journal: Molecular Therapy

Article Title: Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage

doi: 10.1016/j.ymthe.2025.01.038

Figure Lengend Snippet: The macular proteome of the DL-AAA-induced model of chronic retinopathy supports integrin involvement Macular punches including choroid, RPE, and retina obtained at end-study (week 22) were analyzed by mass spectrometry and following analysis using clusterProfiler 4.0 package in R. The proteomes were generated from n = 3 eyes for non-diseased control, n = 5 eyes for DL-AAA treatment, and n = 5 eyes for hAS0326 treatment. (A) Over-representation analysis (ORA) showing the effect of DL-AAA treatment (relative to no DL-AAA treatment) and hAS0326 treatment of DL-AAA-treated eyes (relative to DL-AAA treatment), respectively. The plot was generated using compareCluster function with default settings. All ontologies with significant regulation post correction for multiple testing using the Benjamini-Hochberg procedure are shown. Dot sizes indicate the ratio (i.e., the coverage of a given term by proteins regulated for each comparison), and dot colors indicate the level of significance. (B–E) Volcano plots showing regulation of detected proteins underlying selected GO terms. For selected, significantly regulated gene ontologies, the ORA input proteins displaying significant regulation are highlighted in color. Protein IDs are shown for the top three proteins with lowest p belonging to a particular ontology. Moreover, protein IDs are shown for specific proteins of interest.

Article Snippet: DNA encoding hAS0326 Fab, hAS0326 W33A H-CDR1 Fab, hAS0326 S55A H-CDR2 Fab, hAS0326 H107A H-CDR3, hAS0326 Y32A L-CDR1 Fab, and hAS0326 Y94A L-CDR3 Fab were synthesized and inserted into pcDNA3.1+ plasmids by GenScript.

Techniques: Mass Spectrometry, Generated, Control, Comparison

Journal: Experimental and Therapeutic Medicine

Article Title: Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p

doi: 10.3892/etm.2023.12202

Figure Lengend Snippet: Sequences of primers used for reverse transcription-quantitative PCR.

Article Snippet: CDR1 (cat. no. AB45874; 1:2,000) and β-actin (cat. no. AB21800; 1:2,000) antibodies were purchased from AbSci.

Techniques: Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p

doi: 10.3892/etm.2023.12202

Figure Lengend Snippet: SATB2-AS1 promotes CDR1 and VSNL1 expression in glioma cells. (A-D) Western blotting and reverse transcription-quantitative PCR were performed to detect CDR1 and VSNL1 (A and B) protein expression and (C and D) mRNA expression in U251MG and A172 cells with transfection of pcDNA3.1 or pcDNA3.1-SATB2-AS1. (E and F) By retrieving expression data from TCGA-low grade glioma and TCGA-glioblastoma, the association between SATB2-AS1 and CDR1 or VSNL1 was studied using the Pearson's correlation analysis. * P<0.05, ** P<0.01 and *** P<0.001 vs. control. SATB2-AS1, antisense transcript of SATB2 protein; CDR1, cerebellar degeneration related protein 1; VSNL1, Visinin-like 1; TCGA, The Cancer Genome Atlas.

Article Snippet: CDR1 (cat. no. AB45874; 1:2,000) and β-actin (cat. no. AB21800; 1:2,000) antibodies were purchased from AbSci.

Techniques: Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p

doi: 10.3892/etm.2023.12202

Figure Lengend Snippet: SATB2-AS1 promotes CDR1 and VSNL1 expression by sponging miR-671-5p. (A-D) After transfection of pcDNA3.1 + miR-NC (control group) or pcDNA3.1-SATB2-AS1+ miR-NC (SATB2-AS1 group) or pcDNA3.1-SATB2-AS1 + miR-671-5p mimic (SATB2-AS1 + miR-671-5p group) in U251MG and A172 cells, western blotting and reverse transcription-quantitative PCR were performed to detect CDR1 and VSNL1 (A and B) protein expression and (C and D) mRNA expression in U251MG and A172 cells. ** P<0.01 and *** P<0.001, vs. control (pcDNA3.1 + miR-NC); & P<0.05, && P<0.01 and &&& P<0.001 vs. SATB2-AS1 (pcDNA3.1-SATB2-AS1 + miR-NC). SATB2-AS1, antisense transcript of SATB2 protein; CDR1, cerebellar degeneration related protein 1; VSNL1, Visinin-like 1; miR, microRNA; NC, negative control.

Article Snippet: CDR1 (cat. no. AB45874; 1:2,000) and β-actin (cat. no. AB21800; 1:2,000) antibodies were purchased from AbSci.

Techniques: Expressing, Transfection, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control